catalog number :
MBS013994
products type :
ELISA Kit
products full name :
Hamster Cytochrome P450 7A1 ELISA Kit
products short name :
[Cytochrome P450 7A1]
other names :
[cholesterol 7-alpha-monooxygenase; Cholesterol 7-alpha-monooxygenase; cholesterol 7-alpha-monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1; cytochrome P450, family 7, subfamily A, polypeptide 1; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1]
products gene name :
[CYP7A1]
other gene names :
[CYP7A1; CYP7A1; CP7A; CYP7; CYPVII; CYP7]
uniprot entry name :
CP7A1_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CYP7A1 (hereafter termed "analyte") in undiluted original Hamster body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_000771.2
ncbi gb acc num :
NM_000780.3
ncbi mol weight :
57,661 Da
ncbi pathways :
Bile Acid And Bile Salt Metabolism Pathway (106144); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (413432); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (468297); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Drug Induction Of Bile Acid Pathway (698755); Endogenous Sterols Pathway (105701); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977)
ncbi summary :
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]
uniprot summary :
CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family. Protein type: EC 1.14.13.17; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 8q11-q12. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle. Molecular Function: cholesterol 7-alpha-monooxygenase activity; iron ion binding; heme binding. Biological Process: bile acid biosynthetic process; cholesterol homeostasis; bile acid metabolic process; xenobiotic metabolic process; cellular lipid metabolic process; cholesterol catabolic process; sterol metabolic process; transmembrane transport
size4 :
10x96-Strip-Wells