ELISA/assay

Human Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

MBS013883

48-Strip-Wells

470 USD

ELISA Kit

Human Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

[Cystic Fibrosis Transmembrane Conductance Regulator]

[CFTR, partial; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel]

[CFTR]

[CFTR; CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1; ABCC7; CFTR]

CFTR_HUMAN

Human

This kit recognizes recombinant and natural Human CFTR, no significant cross-reactivity or interference was observed.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 62.5 - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human CFTR in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human CFTR concentration. The concentration of Human CFTR in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

180286

AAA51980.1

P13569

168,142 Da

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Transmembrane Transport Of Small Molecules Pathway (106572)

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice. Protein type: Transporter; Membrane protein, multi-pass; Channel, chloride; Transporter, ABC family; Hydrolase; EC 3.6.3.49; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7q31.2. Cellular Component: recycling endosome; microvillus; cytoplasmic vesicle membrane; cell surface; protein complex; early endosome membrane; basolateral plasma membrane; early endosome; apical plasma membrane; cytoplasm; plasma membrane. Molecular Function: bicarbonate transmembrane transporter activity; chloride channel activity; protein binding; chloride channel inhibitor activity; enzyme binding; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP-binding and phosphorylation-dependent chloride channel activity; ATP binding; PDZ domain binding. Biological Process: intracellular pH elevation; response to drug; response to peptide hormone stimulus; cholesterol transport; iodide transport; transepithelial chloride transport; water transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; vasodilation; cholesterol biosynthetic process; cellular response to hormone stimulus; bicarbonate transport; response to estrogen stimulus; transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development. Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095