catalog number :
MBS013826
products type :
ELISA Kit
products full name :
Canine Thymosin Beta 4 ELISA Kit
products short name :
Thymosin Beta 4
other names :
thymosin beta-4; Thymosin beta-4; thymosin beta-4; t beta-4; prothymosin beta-4; thymosin, beta 4, X chromosome; thymosin beta 4, X-linked; FxCleaved into the following chain:Hematopoietic system regulatory peptide; Alternative name(s):; Seraspenide
products gene name :
Tbeta4
other gene names :
TMSB4X; TMSB4X; FX; TB4X; PTMB4; TMSB4; TB4X; THYB4; TMSB4; T beta-4
uniprot entry name :
TYB4_HUMAN
specificity :
No significant cross-reactivity or interference between Canine Tbeta4 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 ng/ml - 2000 ng/ml. Sensitivity: 10 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated Tbeta4 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine Tbeta4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Tbeta4 and Tbeta4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Tbeta4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated Tbeta4 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine Tbeta4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Tbeta4 and Tbeta4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Tbeta4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_066932.1
ncbi gb acc num :
NM_021109.3
ncbi mol weight :
5,053 Da
ncbi pathways :
Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)
ncbi summary :
This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]
uniprot summary :
TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: Xq21.3-q22. Cellular Component: cytoskeleton; extracellular region. Molecular Function: actin monomer binding; protein binding. Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation