Canine Cx3Cl1/Fractalkine ELISA Kit | MyBioSource MBS013787 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Cx3Cl1/Fractalkine ELISA Kit

catalog :

MBS013787

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS013787

products type :

ELISA Kit

products full name :

Canine Cx3Cl1/Fractalkine ELISA Kit

products short name :

Cx3Cl1/Fractalkine

other names :

CX3CL1; CX3CL1 protein; fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); chemokine (C-X3-C motif) ligand 1

products gene name :

CX3CL1

other gene names :

CX3CL1; CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

uniprot entry name :

Q6I9S9_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine CX3CL1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CX3CL1 and CX3CL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CX3CL1 and CX3CL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

48146969

ncbi acc num :

CAG33707.1

uniprot acc num :

Q6I9S9

ncbi mol weight :

42,187 Da

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114)

uniprot summary :

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; integral to membrane. Molecular Function: protein binding; chemokine activity; receptor binding. Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; angiogenesis involved in wound healing; chemotaxis; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage