catalog number :
MBS013431
products type :
ELISA Kit
products full name :
Bovine Procalcitonin ELISA Kit
products short name :
Procalcitonin
other names :
protein S100-A9; Protein S100-A9; protein S100-A9; p14; MRP-14; calgranulin-B; myeloid-related protein 14; intracellular calcium-binding protein (MRP14); migration inhibitory factor-related protein 14; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9 (calgranulin B); S100 calcium binding protein A9; Calgranulin-B; Migration inhibitory factor-related protein 14; MRP-14; p14; Myeloid-related protein 14; S100 calcium-binding protein A9
other gene names :
S100a9; S100a9; Mrp14; Mrp14; MRP-14; p14
uniprot entry name :
S10A9_RAT
specificity :
No significant cross-reactivity or interference between Bovine PCT and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 50 pg/ml - 1600 pg/ml. Sensitivity: 10 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine PCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCT and PCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine PCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCT and PCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_446039.1
ncbi gb acc num :
NM_053587.1
ncbi mol weight :
13,145 Da
ncbi summary :
calcium binding protein that may be associated with acute inflammatory processes [RGD, Feb 2006]
uniprot summary :
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Calcium-binding. Cellular Component: extracellular space; cytoskeleton; cytoplasm; plasma membrane; nucleus. Molecular Function: antioxidant activity; arachidonic acid binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of peptide secretion; leukocyte migration during inflammatory response; response to ethanol; response to zinc ion; actin cytoskeleton reorganization; innate immune response; autophagy; regulation of integrin biosynthetic process; positive regulation of inflammatory response