ELISA/assay

Human Tissue Polypeptide Antigen ELISA Kit

MBS013396

96 Strip Wells

600 USD

ELISA Kit

Human Tissue Polypeptide Antigen ELISA Kit

Tissue Polypeptide Antigen

tPA, partial; TPA protein; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); plasminogen activator, tissue

TPA

PLAT; tPA; TPA; T-PA

Q6LBF5_HUMAN

Human

19

No significant cross-reactivity or interference between Human TPA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPA concentrations in Human serum, plasma and other body fluids. Using Purified Human TPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPA and TPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detection Range: The detection range of this kit is 15.6U/L-500U/L. Sensitivity: The sensitivity of this kit is 2.0U/L. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPA concentrations in Human serum, plasma and other body fluids. Using Purified Human TPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPA and TPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

2285954

CAA54669.1

Q6LBF5

2,342 Da

Blood Clotting Cascade Pathway 198840!!Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Dissolution Of Fibrin Clot Pathway 106061!!Endochondral Ossification Pathway 198812!!Hemostasis Pathway 106028!!Selenium Pathway 198825!!Senescence And Autophagy Pathway 198780!!Signal Transduction Pathway 477114

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

96 Strip Wells

600 USD