ELISA/assay

Rat Histamine ELISA Kit

MBS013382

48-Strip-Wells

435 USD

ELISA Kit

Rat Histamine ELISA Kit

Histamine

histamine H2 receptor isoform 1; Histamine H2 receptor; histamine H2 receptor; HH2R; gastric receptor 1; gastric receptor I; histamine receptor H2; Gastric receptor I

HIS

HRH2; HRH2; H2R; H2R; HH2R

HRH2_HUMAN

Rat

397

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma and Tissue Homogenate. Assay Type: Sandwich. Detection Range: 1.25 ng/ml - 40 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of HIS (hereafter termed this analyte) in undiluted original Rat serum, plasma and tissue homogenate samples.

197085610

NP_001124527.1

NM_001131055.1

P25021

40,098 Da

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383)

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 5q35.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: histamine receptor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; gland development; gastrin-induced gastric acid secretion; histamine-induced gastric acid secretion; gut development; positive regulation of vasoconstriction; visual learning; regulation of synaptic plasticity; immune response; memory

48-Strip-Wells

435 USD

96-Strip-Wells

600