ELISA/assay

Human Apoptotic Peptidase Activating Factor 1 ELISA Kit

MBS012316

96 Strip Wells

600 USD

ELISA Kit

Human Apoptotic Peptidase Activating Factor 1 ELISA Kit

Apoptotic Peptidase Activating Factor 1

APAF1 protein; Apoptotic protease-activating factor 1; apoptotic protease-activating factor 1; apoptotic peptidase activating factor 1

APAF1

APAF1; APAF1; CED4; APAF-1; KIAA0413; APAF-1

APAF_HUMAN

Human

Store all reagents at 2-8 degree C

ELISA Type: Sandwich

148342475

ABQ59028.1

O14727

141,840 Da

Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway 105672!!Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Ref.4 Ref.5. Subunit structure: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Ref.4 Ref.12 Ref.14 Ref.16. Subcellular location: Cytoplasm Ref.5. Tissue specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. Induction: By E2F and p53/TP53 in apoptotic neurons. Ref.13. Domain: The CARD domain mediates interaction with APIP.The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization . By similarity. Miscellaneous: Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form. Sequence similarities: Contains 1 CARD domain.Contains 1 NB-ARC domain.Contains 15 WD repeats. Sequence caution: The sequence AAK28401.1 differs from that shown. Reason: Frameshift at position 108. The sequence BAA24843.2 differs from that shown. Reason: Erroneous initiation.

96 Strip Wells

600 USD