ELISA/assay

Human Acetylcholinesterase ELISA Kit

MBS012071

96 Strip Wells

600 USD

ELISA Kit

Human Acetylcholinesterase ELISA Kit

Acetylcholinesterase

acetylcholinesterase; Acetylcholinesterase; acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase; acetylcholinesterase

ACHE

ACHE; ACHE; YT; ACEE; ARACHE; N-ACHE; AChE

ACES_HUMAN

Human

No significant cross-reactivity or interference between Human ACHE and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACHE concentrations in Human serum, plasma and other body fluids. Using Purified Human ACHE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACHE and ACHE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACHE in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 12.5U/L-400U/L.

Sensitivity: The sensitivity of this kit is 2.0U/L. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACHE concentrations in Human serum, plasma and other body fluids. Using Purified Human ACHE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACHE and ACHE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACHE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

545225

AAC60618.1

P22303

67,796 Da

ATF-2 Transcription Factor Network Pathway 138006!!Acetylcholine Synthesis Pathway 198820!!Biogenic Amine Synthesis Pathway 198793!!Cholinergic Synapse Pathway 217716!!Glycerophospholipid Biosynthesis Pathway 645313!!Glycerophospholipid Metabolism Pathway 82989!!Glycerophospholipid Metabolism Pathway 364!!Integrated Pancreatic Cancer Pathway 711360!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Ref.11 Ref.12 Ref.13 Ref.14. Catalytic activity: Acetylcholine + H2O = choline + acetate. Subunit structure: Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers . By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric. Subcellular location: Cell junction synapse. Secreted . By similarity. Cell membrane; Peripheral membrane protein . By similarity Ref.12 Ref.14. Isoform T: Nucleus. Note: Only observed in apoptotic nuclei. Ref.12 Ref.14Isoform H: Cell membrane; Lipid-anchor GPI-anchor; Extracellular side . By similarity Ref.12 Ref.14. Tissue specificity: Isoform H is highly expressed in erythrocytes. Ref.11. Polymorphism: ACHE is responsible for the Yt blood group system [. MIM:112100]. The molecular basis of the Yt(a)=Yt1/Yt(b)=Yt2 blood group antigens is a single variation in position 353; His-353 corresponds to Yt(a) and the rare variant with Asn-353 to Yt(b). Sequence similarities: Belongs to the type-B carboxylesterase/lipase family.

96 Strip Wells

600 USD