ELISA/assay

Rat Glypican 3 ELISA Kit

MBS012057

48-Strip-Wells

435 USD

ELISA Kit

Rat Glypican 3 ELISA Kit

Glypican 3

glypican-3 isoform 4; Glypican-3; glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7

GPC3

GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5

GPC3_HUMAN

Rat

No significant cross-reactivity or interference between Rat GPC3 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GPC3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPC3 and GPC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GPC3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPC3 and GPC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

257471010

NP_001158091.1

NM_001164619.1

P51654

65,563 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Glycosaminoglycan Metabolism Pathway (645297); Glypican 3 Network Pathway (138084); Glypican Pathway (138083); HS-GAG Biosynthesis Pathway (645306); HS-GAG Degradation Pathway (645307); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (645304)

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family. Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: Xq26.1. Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; protein binding. Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; positive regulation of endocytosis; pathogenesis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; positive regulation of glucose import; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of growth; anterior/posterior axis specification; lung development. Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

48-Strip-Wells

435 USD

96-Strip-Wells

600