ELISA/assay

Rabbit Granzymes B ELISA Kit

MBS011732

48-Strip-Wells

435 USD

ELISA Kit

Rabbit Granzymes B ELISA Kit

Granzymes B

granzyme B; Granzyme B; granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

GZMS-B

GZMB; GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

GRAB_HUMAN

Rabbit

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Urine and other Body Fluids. Detection Range: 3.12 pg/ml ~ 100 pg/ml.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit GZMS-B in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rabbit GZMS-B concentration. The concentration of Rabbit GZMS-B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rabbit GZMS-B in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rabbit GZMS-B concentration. The concentration of Rabbit GZMS-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

221625528

NP_004122.2

NM_004131.4

P10144

27,716 Da

Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway (105657); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Graft-versus-host Disease Pathway (83124)

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Protease; Apoptosis; EC 3.4.21.79. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; cytosol; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; natural killer cell mediated cytotoxicity; apoptosis; cytolysis; immune response; protein processing; proteolysis

48-Strip-Wells

435 USD

96-Strip-Wells

600