Rat Cathepsin S ELISA Kit | MyBioSource MBS011479 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Cathepsin S ELISA Kit

catalog :

MBS011479

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS011479

products type :

ELISA Kit

products full name :

Rat Cathepsin S ELISA Kit

products short name :

Cathepsin S

other names :

cathepsin S isoform 2 preproprotein; Cathepsin S; cathepsin S; cathepsin S

products gene name :

CTSS

other gene names :

CTSS; CTSS

uniprot entry name :

CATS_HUMAN

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between Rat CTSS and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTSS concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTSS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSS and CTSS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTSS concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTSS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSS and CTSS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

315075311

ncbi acc num :

NP_001186668.1

ncbi gb acc num :

NM_001199739.1

uniprot acc num :

P25774

ncbi mol weight :

37,496 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Collagen Formation Pathway (645288); Endosomal/Vacuolar Pathway (477125); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386)

ncbi summary :

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

uniprot summary :

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.22.27; Protease. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; lysosomal lumen; intracellular membrane-bound organelle; membrane; lysosome; extracellular region. Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding. Biological Process: extracellular matrix organization and biogenesis; adaptive immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; antigen processing and presentation; extracellular matrix disassembly; collagen catabolic process; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage