ELISA/assay

Human Semaphorin-4D ELISA Kit

MBS011111

48-Strip-Wells

435 USD

ELISA Kit

Human Semaphorin-4D ELISA Kit

Semaphorin-4D

semaphorin-4D isoform 1; Semaphorin-4D; semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; A8; BB18; GR3

SEMA4D

SEMA4D; SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G; C9orf164; CD100; SEMAJ

SEM4D_HUMAN

Human

No significant cross-reactivity or interference between Human SEMA4D and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMA4D concentrations in Human serum, plasma and other body fluids. Using Purified Human SEMA4D antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMA4D and SEMA4D antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMA4D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

214010218

NP_006369.3

NM_006378.3

Q92854

96,150 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Other Semaphorin Interactions Pathway (119526); Sema4D In Semaphorin Signaling Pathway (119520); Sema4D Induced Cell Migration And Growth-cone Collapse Pathway (119522); Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway (119521); Semaphorin Interactions Pathway (119519)

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9q22.2. Cellular Component: extracellular space; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding. Biological Process: axon guidance; negative regulation of peptidyl-tyrosine phosphorylation; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; immune response; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; regulation of cell projection organization and biogenesis; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells

435 USD

96-Strip-Wells

600