ELISA/assay

Human Serum Albumin ELISA Kit

MBS010908

96 Strip Wells

600 USD

ELISA Kit

Human Serum Albumin ELISA Kit

Serum Albumin

serum albumin preproprotein; Serum albumin; serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42; albumin

HSA

ALB; ALB; PRO0883; PRO0903; PRO1341

ALBU_HUMAN

Human

No significant cross-reactivity or interference between Human SA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SA concentrations in Human serum, plasma and other body fluids. Using Purified Human SA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SA and SA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 5 mg/ml - 160 mg/ml.

Sensitivity: The sensitivity of this kit is 1.0 mg/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SA concentrations in Human serum, plasma and other body fluids. Using Purified Human SA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SA and SA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4502027

NP_000468.1

NM_000477.5

P02768

69,367 Da

Bile Acid And Bile Salt Metabolism Pathway 106144!!Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!HDL-mediated Lipid Transport Pathway 106158!!Hemostasis Pathway 106028!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Platelet Activation, Signaling And Aggregation Pathway 106034

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Ref.32. Subcellular location: Secreted. Tissue specificity: Plasma. Post-translational modification: Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.Glycated in diabetic patients.Phosphorylation sites are present in the extracellular medium.Acetylated on Lys-223 by acetylsalicylic acid. Polymorphism: A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. The sequence shown is that of variant albumin A. Involvement in disease: Familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]: Form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.61 Ref.62 Ref.63 Ref.64. Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains. Caution: A peptide arising from positions 166 to 174 was originally (Ref.23 and Ref.24) termed neurotensin-related peptide (NRP) or kinetensin and was thought to regulate fat digestion, lipid absorption, and blood flow. Sequence caution: The sequence AAF22034.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAF69644.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAG35503.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

96 Strip Wells

600 USD