ELISA/assay

Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

MBS010824

48-Strip-Wells

435 USD

ELISA Kit

Porcine Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit

Cystic Fibrosis Transmembrane Conductance Regulator

CFTR, partial; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel

CFTR

CFTR; CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1; ABCC7; CFTR

CFTR_HUMAN

Porcine

No significant cross-reactivity or interference between Porcine CFTR and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFTR concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CFTR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFTR and CFTR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFTR in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFTR concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine CFTR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFTR and CFTR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFTR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

180286

AAA51980.1

P13569

168,142 Da

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Transmembrane Transport Of Small Molecules Pathway (106572)

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice. Protein type: Hydrolase; Membrane protein, integral; Transporter, ABC family; Transporter; Channel, chloride; EC 3.6.3.49; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 7q31.2. Cellular Component: recycling endosome; protein complex; microvillus; cytoplasmic vesicle membrane; cell surface; early endosome membrane; basolateral plasma membrane; early endosome; apical plasma membrane; cytoplasm; plasma membrane. Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; enzyme binding; chloride channel inhibitor activity; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP-binding and phosphorylation-dependent chloride channel activity; ATP binding; PDZ domain binding. Biological Process: response to drug; intracellular pH elevation; response to peptide hormone stimulus; cholesterol transport; iodide transport; water transport; transepithelial chloride transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; cellular response to hormone stimulus; bicarbonate transport; response to estrogen stimulus; transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development. Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary

48-Strip-Wells

435 USD

96-Strip-Wells

600