ELISA/assay

Human Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

MBS010804

96 Strip Wells

600 USD

ELISA Kit

Human Glyceraldehyde 3 Phosphate Dehydrogenase ELISA Kit

Glyceraldehyde 3 Phosphate Dehydrogenase

Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH

GAPDH

Gapdh; Gapdh; Gapd; Gapd; GAPDH

G3P_RAT

Human

Store all reagents at 2-8 degree C

ELISA Type: Sandwich

122065190

P04797.3

P04797

35,828 Da

Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Androgen Receptor Signaling Pathway 198529!!Biosynthesis Of Amino Acids Pathway 791250!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 816129!!Carbon Metabolism Pathway 817567!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 434661!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 468196!!Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate 434659

catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD, Feb 2006]

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation . By similarity. Ref.3 Ref.12 Ref.13 Ref.15. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct . By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules. Component of the GAIT complex . By similarity. Ref.12 Ref.13 Ref.14 Ref.15. Subcellular location: Cytoplasm cytosol. Cytoplasm cytoskeleton. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the microtubules tracks. Ref.12 Ref.13 Ref.14 Ref.15. Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.8. Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.11). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated . By similarity.Sulfhydration at Cys-150 increases catalytic activity . By similarity. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

96 Strip Wells

600 USD