Horse Stromal Cell Derived Factor 1alpha ELISA Kit | MyBioSource MBS010582 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Horse Stromal Cell Derived Factor 1alpha ELISA Kit

catalog :

MBS010582

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS010582

products type :

ELISA Kit

products full name :

Horse Stromal Cell Derived Factor 1alpha ELISA Kit

products short name :

[Stromal Cell Derived Factor 1alpha]

other names :

[stromal cell-derived factor 1 isoform gamma; Stromal cell-derived factor 1; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor]

products gene name :

[SDF1alpha]

other gene names :

[CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF]

uniprot entry name :

SDF1_HUMAN

reactivity :

Horse

sequence length :

119

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Undiluted original Horse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of SDF1A (hereafter termed "analyte") in undiluted original Horse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

76563933

ncbi acc num :

NP_001029058.1

ncbi gb acc num :

NM_001033886.2

uniprot acc num :

P48061

ncbi mol weight :

10,666 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550)

ncbi summary :

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

uniprot summary :

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell development/differentiation; Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.1. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: growth factor activity; chemokine activity; chemokine receptor binding; CXCR chemokine receptor binding; receptor binding. Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; signal transduction; chemotaxis; germ cell development; induction of positive chemotaxis; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to heat; response to mechanical stimulus; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; positive regulation of endothelial cell proliferation; immune response; telencephalon cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage