Canine Interleukin 7 ELISA Kit | MyBioSource MBS010391 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Interleukin 7 ELISA Kit

catalog :

MBS010391

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS010391

products type :

ELISA Kit

products full name :

Canine Interleukin 7 ELISA Kit

products short name :

Interleukin 7

other names :

interleukin-7 isoform 4; Interleukin-7; interleukin-7; interleukin 7

products gene name :

IL-7

other gene names :

IL7; IL7; IL-7; IL-7

uniprot entry name :

IL7_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine IL-7 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-7 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-7 and IL-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-7 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-7 and IL-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

315467871

ncbi acc num :

NP_001186817.1

ncbi gb acc num :

NM_001199888.1

uniprot acc num :

P13232

ncbi mol weight :

20,187 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Cytokine Signaling In Immune System Pathway (819682); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (819567); Immune System Pathway (106386); Interleukin-7 Signaling Pathway (819698); Interleukin-7 Signaling Pathway (530762)

ncbi summary :

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

uniprot summary :

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell development/differentiation; Cytokine; Secreted, signal peptide; Apoptosis; Cell cycle regulation. Chromosomal Location of Human Ortholog: 8q12-q13. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity. Biological Process: organ morphogenesis; T cell lineage commitment; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of organ growth; humoral immune response; negative regulation of apoptosis; bone resorption

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage