Mouse Vasoactive Intestinal Peptide ELISA Kit | MyBioSource MBS010053 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Vasoactive Intestinal Peptide ELISA Kit

catalog :

MBS010053

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS010053

products type :

ELISA Kit

products full name :

Mouse Vasoactive Intestinal Peptide ELISA Kit

products short name :

Vasoactive Intestinal Peptide

other names :

VIP peptides isoform 1 preproprotein; VIP peptides; VIP peptides; prepro-VIP; vasoactive intestinal peptide; Peptide histidine valine 42Intestinal peptide PHM-27; Alternative name(s):; Peptide histidine methioninamide 27Vasoactive intestinal peptide; VIP; Alternative name(s):; Vasoactive intestinal polypeptide

products gene name :

VIP

other gene names :

VIP; VIP; PHM27; VIP

uniprot entry name :

VIP_HUMAN

reactivity :

Mouse

sequence length :

170

specificity :

No significant cross-reactivity or interference between Mouse VIP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VIP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse VIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VIP and VIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VIP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse VIP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VIP and VIP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4507897

ncbi acc num :

NP_003372.1

ncbi gb acc num :

NM_003381.3

uniprot acc num :

P01282

ncbi mol weight :

19,169 Da

ncbi pathways :

G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6q25. Cellular Component: cell soma; extracellular region. Molecular Function: neuropeptide hormone activity; protein binding; hormone activity. Biological Process: negative regulation of smooth muscle cell proliferation; negative regulation of potassium ion transport; mRNA stabilization; regulation of signal transduction; positive regulation of vasodilation; body fluid secretion; G-protein coupled receptor protein signaling pathway; regulation of protein localization; learning and/or memory; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of endothelial cell proliferation; regulation of sensory perception of pain; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage