ELISA/assay

Rat Neuroglobin ELISA Kit

MBS010027

48-Strip-Wells

435 USD

ELISA Kit

Rat Neuroglobin ELISA Kit

Neuroglobin

neuroglobin; Neuroglobin; neuroglobin; neuroglobin

NGB

NGB; NGB

NGB_HUMAN

Rat

No significant cross-reactivity or interference between Rat NGB and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 mumol/L - 8 mumol/L. Sensitivity: 0.1 mumol/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGB concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NGB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGB and NGB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGB concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NGB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGB and NGB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10864065

NP_067080.1

NM_021257.3

Q9NPG2

16,933 Da

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]

NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: mitochondrion; perikaryon. Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity. Biological Process: oxygen transport; apoptosis

48-Strip-Wells

435 USD

96-Strip-Wells

600