Porcine Complement Fragment 3A ELISA Kit | MyBioSource MBS009647 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Complement Fragment 3A ELISA Kit

catalog :

MBS009647

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS009647

products type :

ELISA Kit

products full name :

Porcine Complement Fragment 3A ELISA Kit

products short name :

Complement Fragment 3A

other names :

C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; complement component 3a receptor 1

products gene name :

C3A

other gene names :

C3ar1; C3ar1; C3AR; C3a-R

uniprot entry name :

C3AR_RAT

reactivity :

Porcine

sequence length :

473

specificity :

No significant cross-reactivity or interference between Porcine C3A and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C3A concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine C3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3A and C3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated C3A concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine C3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add C3A and C3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

14091740

ncbi acc num :

NP_114449.1

ncbi gb acc num :

NM_032060.1

uniprot acc num :

O55197

ncbi mol weight :

52,896 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); Complement And Coagulation Cascades Pathway (198461); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484); G Alpha (i) Signalling Events Pathway (1010195); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); GPCRs, Class A Rhodopsin-like Pathway (198500); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

receptor for anaphylatoxin complement component C3a, [RGD, Feb 2006]

uniprot summary :

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral. Cellular Component: plasma membrane; integral to membrane. Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a binding. Biological Process: tolerance induction to nonself antigen; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; positive regulation of smooth muscle cell proliferation; regulation of blood pressure; complement receptor mediated signaling pathway; chemotaxis; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage