Fish Thioredoxin ELISA Kit | MyBioSource MBS009625 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Thioredoxin ELISA Kit

catalog :

MBS009625

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS009625

products type :

ELISA Kit

products full name :

Fish Thioredoxin ELISA Kit

products short name :

Thioredoxin

other names :

thioredoxin; Thioredoxin; thioredoxin; trx; ATL-derived factor; thioredoxin 1; ATL-derived factor

products gene name :

TRX

other gene names :

Txn1; Txn; ADF; Txn; Trx1; AW550880; Txn1; Trx; ADF

uniprot entry name :

THIO_MOUSE

reactivity :

Fish

specificity :

No significant cross-reactivity or interference between Fish TRX and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRX concentrations in Fish serum, plasma and other body fluids. Using Purified Fish TRX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRX and TRX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRX in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRX concentrations in Fish serum, plasma and other body fluids. Using Purified Fish TRX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRX and TRX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6755911

ncbi acc num :

NP_035790.1

ncbi gb acc num :

NM_011660.3

uniprot acc num :

P10639

ncbi mol weight :

11,675 Da

ncbi pathways :

Immune System Pathway (819567); Inflammasomes Pathway (819646); Innate Immune System Pathway (819596); Metabolism Pathway (819200); Metabolism Of Nucleotides Pathway (819361); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (819644); Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway (819370); The NLRP3 Inflammasome Pathway (819647)

uniprot summary :

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family. Protein type: Nuclear receptor co-regulator. Cellular Component: mitochondrion; cell soma; axon; dendrite; cytoplasm; extracellular region; cytosol; nucleus. Molecular Function: protein binding; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor. Biological Process: sulfate assimilation; response to radiation; negative regulation of protein export from nucleus; regulation of transcription, DNA-dependent; transcription, DNA-dependent; protein folding; transport; cell redox homeostasis; regulation of protein import into nucleus, translocation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of DNA binding

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage