Human Activating Transcription Factor 3 ELISA Kit | MyBioSource MBS009530 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Activating Transcription Factor 3 ELISA Kit

catalog :

MBS009530

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS009530

products type :

ELISA Kit

products full name :

Human Activating Transcription Factor 3 ELISA Kit

products short name :

Activating Transcription Factor 3

other names :

ATF3; Cyclic AMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; activating transcription factor 3; Activating transcription factor 3

products gene name :

ATF3

other gene names :

ATF3; ATF3; cAMP-dependent transcription factor ATF-3

uniprot entry name :

ATF3_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human ATF3 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ATF3 concentrations in Human serum, plasma and other body fluids. Using Purified Human ATF3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ATF3 and ATF3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ATF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ATF3 concentrations in Human serum, plasma and other body fluids. Using Purified Human ATF3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ATF3 and ATF3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ATF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

47496615

ncbi acc num :

CAG29330.1

uniprot acc num :

Q6ICQ9

ncbi mol weight :

20,576 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Activation Of Genes By ATF4 Pathway (530772); Direct P53 Effectors Pathway (137939); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Id Signaling Pathway (198871); Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); PERK Regulated Gene Expression Pathway (105907); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963)

ncbi summary :

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

uniprot summary :

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5 -GTGACGT[AC][AG]-3 ), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor. Chromosomal Location of Human Ortholog: 1q32.3. Cellular Component: nucleoplasm; nucleolus; nucleus. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity. Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage