catalog number :
MBS009193
products type :
ELISA Kit
products full name :
Rat Indoleamine 2,3-Dioxygenase ELISA Kit
products short name :
[Indoleamine 2,3-Dioxygenase]
other names :
[Indoleamine 2,3-dioxygenase; Indoleamine 2,3-dioxygenase; Bna2p; Biosynthesis of nicotinic acid protein 2]
products gene name :
[IDO]
other gene names :
[BNA2; BNA2; IDO]
uniprot entry name :
I23O_YEAST
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Human Body Fluids, Tissue Homogenates, Secretions Or Feces Samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625mmol/L-20mmol/L. Sensitivity: 0.1mmol/L.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of IDL (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi mol weight :
50,775 Da
ncbi pathways :
De Novo NAD Biosynthesis Pathway (198675); Metabolism Pathway (829779); Metabolism Of Amino Acids And Derivatives Pathway (829897); NAD Biosynthesis II (from Tryptophan) Pathway (143526); NAD Biosynthesis II (from Tryptophan) Pathway (138368); Tryptophan Degradation Via Kynurenine Pathway (198749); Tryptophan Catabolism Pathway (829904); Tryptophan Metabolism Pathway (889); Tryptophan Metabolism Pathway (332); Superpathway Of NAD Biosynthesis In Eukaryotes (138369)
uniprot summary :
Function: Catalyzes the first step in tryptophan catabolism in order to supply de novo nicotinamide adenine dinucleotide (NAD+) via the kynurenine pathway. Plays a role in the cellular response to telomere uncapping. Catalytic activity: D-tryptophan + O2 = N-formyl-D-kynurenine.L-tryptophan + O2 = N-formyl-L-kynurenine. Cofactor: Binds 1 heme group per subunit . By similarity. Pathway: Cofactor biosynthesis; NAD(+) biosynthesis. Ref.4. Induction: Expression is mediated by the AFT2, HCM1, and SUM1 transcription factors. Up-regulated in absence of NPT1. Miscellaneous: Present with 149 molecules/cell in log phase SD medium. Sequence similarities: Belongs to the indoleamine 2,3-dioxygenase family. Biophysicochemical propertiesKinetic parameters:KM=412 uM for L-tryptophanKM=9.2 mM for D-tryptophan
size4 :
10x96-Strip-Wells