ELISA/assay

Human Resistin ELISA Kit

MBS008924

96 Strip Wells

600 USD

ELISA Kit

Human Resistin ELISA Kit

Resistin

resistin; Resistin; resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3

RESISTIN

RETN; RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1; FIZZ3; HXCP1; RSTN; ADSF

RETN_HUMAN

Human

No significant cross-reactivity or interference between Human RESISTIN and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RESISTIN concentrations in Human serum, plasma and other body fluids. Using Purified Human RESISTIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RESISTIN and RESISTIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RESISTIN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 1.56 ng/ml - 50 ng/ml.

Sensitivity: The sensitivity of this kit is 0.1 ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RESISTIN concentrations in Human serum, plasma and other body fluids. Using Purified Human RESISTIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RESISTIN and RESISTIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RESISTIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

12584202

AAG59824.1

Q9HD89

11,419 Da

Adipogenesis Pathway 198832

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Subunit structure: Homodimer; disulfide-linked . By similarity. Subcellular location: Secreted. Tissue specificity: Expressed only in fatty tissues. Sequence similarities: Belongs to the resistin/FIZZ family.

96 Strip Wells

600 USD