Human Platelet Factor 4 ELISA Kit | MyBioSource MBS008893 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Platelet Factor 4 ELISA Kit

catalog :

MBS008893

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS008893

products type :

ELISA Kit

products full name :

Human Platelet Factor 4 ELISA Kit

products short name :

Platelet Factor 4

other names :

platelet factor 4; Platelet factor 4; platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; platelet factor 4; C-X-C motif chemokine 4; Iroplact; Oncostatin-A

products gene name :

PF4

other gene names :

PF4; PF4; PF-4; CXCL4; SCYB4; CXCL4; SCYB4; PF-4

uniprot entry name :

PLF4_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human PF4 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PF4 concentrations in Human serum, plasma and other body fluids. Using Purified Human PF4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PF4 and PF4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505733

ncbi acc num :

NP_002610.1

ncbi gb acc num :

NM_002619.3

uniprot acc num :

P02776

ncbi mol weight :

10,845 Da

ncbi pathways :

CXCR3-mediated Signaling Events Pathway (138011); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057)

ncbi summary :

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

uniprot summary :

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Chemokine; Motility/polarity/chemotaxis; Apoptosis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q21. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity. Biological Process: negative regulation of cytolysis; platelet activation; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of macrophage differentiation; blood coagulation

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage