ELISA/assay

Bovine Alpha B-Crystallin ELISA Kit

MBS008260

48-Strip-Wells

435 USD

ELISA Kit

Bovine Alpha B-Crystallin ELISA Kit

Alpha B-Crystallin

CRYAB; Alpha-crystallin B chain; crystallin, alpha B; Alpha(B)-crystallin; Alpha-crystallin B chain; Alpha(B)-crystallin

CRYAB

CRYAB; CRYAB

CRYAB_PIG

Bovine

No significant cross-reactivity or interference between Bovine CRYAB and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CRYAB concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine CRYAB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRYAB and CRYAB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRYAB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CRYAB concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine CRYAB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRYAB and CRYAB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRYAB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

169882027

ACA97071.1

Q7M2W6

20,129 Da

Protein Processing In Endoplasmic Reticulum Pathway (167312); Protein Processing In Endoplasmic Reticulum Pathway (167190)

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Protein type: Heat shock protein; Chaperone. Cellular Component: mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; Z disc. Molecular Function: protein homodimerization activity; metal ion binding; unfolded protein binding; structural constituent of eye lens. Biological Process: lens development in camera-type eye; tubulin folding; muscle development; negative regulation of intracellular transport; response to hydrogen peroxide; response to hypoxia; negative regulation of caspase activity; protein homooligomerization

48-Strip-Wells

435 USD

96-Strip-Wells

600