Hamster Acid Sphingomyelinase ELISA Kit | MyBioSource MBS008000 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hamster Acid Sphingomyelinase ELISA Kit

catalog :

MBS008000

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS008000

products type :

ELISA Kit

products full name :

Hamster Acid Sphingomyelinase ELISA Kit

products short name :

Acid Sphingomyelinase

other names :

acid sphingomyelinase; Sphingomyelin phosphodiesterase; sphingomyelin phosphodiesterase; acid sphingomyelinase; sphingomyelin phosphodiesterase 1, acid lysosomal; Acid sphingomyelinase

products gene name :

ASM

other gene names :

SMPD1; SMPD1; ASM; NPD; ASMASE; ASM; aSMase

uniprot entry name :

ASM_HUMAN

reactivity :

Hamster

specificity :

No significant cross-reactivity or interference between Hamster ASM and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 U/L - 200 U/L. Sensitivity: 1.0 U/L.

other info2 :

Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate ASM concentrations in Hamster serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate ASM concentrations in Hamster serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!

ncbi gi num :

179095

ncbi acc num :

AAA58377.1

uniprot acc num :

P17405

ncbi mol weight :

69,752 Da

ncbi pathways :

Ceramide Signaling Pathway (138023); FAS (CD95) Signaling Pathway (138081); Glycosphingolipid Metabolism Pathway (530751); IL2 Signaling Events Mediated By PI3K Pathway (137938); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Infertility Genes Pathway (198801); Sphingolipid Metabolism Pathway (82994)

ncbi summary :

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

uniprot summary :

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; EC 3.1.4.12. Chromosomal Location of Human Ortholog: 11p15.4-p15.1. Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body. Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds. Biological Process: response to drug; nervous system development; negative regulation of MAP kinase activity; sphingomyelin metabolic process; sphingolipid metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation. Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage