Mouse Apolipoprotein C3 ELISA Kit | MyBioSource MBS007937 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein C3 ELISA Kit

catalog :

MBS007937

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS007937

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein C3 ELISA Kit

products short name :

Apolipoprotein C3

other names :

apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III; Apolipoprotein C3

products gene name :

APOC3

other gene names :

APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III

uniprot entry name :

APOC3_HUMAN

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse APOC3 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 2.5 mg/dl - 80 mg/dl. Sensitivity: 0.5 mg/dl.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APOC3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APOC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOC3 and APOC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated APOC3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APOC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOC3 and APOC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4557323

ncbi acc num :

NP_000031.1

ncbi gb acc num :

NM_000040.1

uniprot acc num :

P02656

ncbi mol weight :

10,852 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Secreted; Secreted, signal peptide; Lipid-binding. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity. Biological Process: response to drug; cholesterol metabolic process; response to peptide hormone stimulus; phototransduction, visible light; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; cholesterol efflux; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; negative regulation of lipid metabolic process; retinoid metabolic process; inflammatory response; triacylglycerol mobilization; negative regulation of fatty acid biosynthetic process; response to nutrient. Disease: Apolipoprotein C-iii Deficiency

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage