ELISA/assay

Human E3/Ubiquitin-Protein Ligase ELISA Kit

MBS007911

48-Strip-Wells

435 USD

ELISA Kit

Human E3/Ubiquitin-Protein Ligase ELISA Kit

E3/Ubiquitin-Protein Ligase

E3 ubiquitin-protein ligase UBR5 isoform 2; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase UBR5; progestin induced protein; progestin-induced protein; hyperplastic discs protein homolog; E3 identified by differential display; E3 ubiquitin-protein ligase, HECT domain-containing 1; E3 ubiquitin protein ligase, HECT domain containing, 1; ubiquitin protein ligase E3 component n-recognin 5; E3 ubiquitin-protein ligase, HECT domain-containing 1; Hyperplastic discs protein homolog; hHYD; Progestin-induced protein

E3/UBPL

UBR5; UBR5; DD5; EDD; HYD; EDD1; EDD; EDD1; HYD; KIAA0896; hHYD

UBR5_HUMAN

Human

2798

No significant cross-reactivity or interference between Human E3/UBPL and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated E3/UBPL concentrations in Human serum, plasma and other body fluids. Using Purified Human E3/UBPL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add E3/UBPL and E3/UBPL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E3/UBPL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated E3/UBPL concentrations in Human serum, plasma and other body fluids. Using Purified Human E3/UBPL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add E3/UBPL and E3/UBPL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E3/UBPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

544583486

NP_001269802.1

NM_001282873.1

O95071

309,352 Da

Tryptophan Metabolism Pathway (198850); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. Protein type: EC 6.3.2.19; Nuclear receptor co-regulator; Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-. Chromosomal Location of Human Ortholog: 8q22. Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus. Molecular Function: protein binding; ubiquitin binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; progesterone receptor signaling pathway; protein polyubiquitination; positive regulation of protein import into nucleus, translocation; protein ubiquitination during ubiquitin-dependent protein catabolic process; DNA repair; response to DNA damage stimulus

48-Strip-Wells

435 USD

96-Strip-Wells

600