ELISA/assay

Hamster Peroxisome Proliferators Activated Receptor alpha ELISA Kit

MBS007846

48-Strip-Wells

435 USD

ELISA Kit

Hamster Peroxisome Proliferators Activated Receptor alpha ELISA Kit

Peroxisome Proliferators Activated Receptor alpha

peroxisome proliferator-activated receptor alpha; Peroxisome proliferator-activated receptor alpha; peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; Nuclear receptor subfamily 1 group C member 1

PPARalpha

PPARA; PPARA; PPAR; NR1C1; hPPAR; PPARalpha; NR1C1; PPAR; PPAR-alpha

PPARA_HUMAN

Hamster

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 62.5 pg/ml ~ 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Hamster PPARalpha in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Hamster PPARalpha concentration. The concentration of Hamster PPARalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

50348666

NP_001001928.1

NM_001001928.2

Q07869

52,225 Da

Activation Of Gene Expression By SREBP (SREBF) Pathway (685552); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); Circadian Repression Of Expression By REV-ERBA Pathway (576259); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977)

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; NFAT protein binding; zinc ion binding; drug binding; phosphatase binding; protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; transcription factor activity; lipid binding. Biological Process: circadian rhythm; epidermis development; wound healing; heart development; positive regulation of transcription, DNA-dependent; behavioral response to nicotine; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of protein binding; negative regulation of glycolysis; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; fatty acid transport

48-Strip-Wells

435 USD

96-Strip-Wells

600