ELISA/assay

Rat Lctin Like Oxldl Receptor 1 ELISA Kit

MBS007372

48-Strip-Wells

435 USD

ELISA Kit

Rat Lctin Like Oxldl Receptor 1 ELISA Kit

Lctin Like Oxldl Receptor 1

lectin-like oxidized low-density lipoprotein receptor; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density lipoprotein receptor-1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1

LOX-1

Olr1; Olr1; LOX-1; Oldr1; Oldlr1; Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1

OLR1_RAT

Rat

364

No significant cross-reactivity or interference between Rat LOX-1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LOX-1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat LOX-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LOX-1 and LOX-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LOX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LOX-1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat LOX-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LOX-1 and LOX-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LOX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4008187

BAA35123.1

O70156

41,890 Da

Cell Surface Interactions At The Vascular Wall Pathway (1010919); Hemostasis Pathway (1010891); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450); Phagosome Pathway (153901); Phagosome Pathway (153859)

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.2 Ref.4. Subunit structure: Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70 . By similarity. Subcellular location: Cell membrane; Lipid-anchor . By similarity. Cell membrane; Single-pass type II membrane protein . By similarity. Membrane raft . By similarity. Secreted . By similarity. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation . By similarity. Tissue specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells. Ref.1. Induction: By hypertension. Up-regulated by shear stress, lipopolysaccharide and TNF-alpha in cultured vascular endothelial cells. Ref.1 Ref.2. Domain: The cytoplasmic region is required for subcellular sorting on the cell surface . By similarity.The C-type lectin domain mediates the recognition and binding of oxLDL . By similarity.The Neck region contains 3 internal repeats that are only found in rodents. Post-translational modification: N-glycosylated . By similarity. Sequence similarities: Contains 1 C-type lectin domain.

48-Strip-Wells

435 USD

96-Strip-Wells

600