ELISA/assay

Rat Nitric Oxide Synthase ELISA Kit

MBS007234

96 Strip Wells

600 USD

ELISA Kit

Rat Nitric Oxide Synthase ELISA Kit

Nitric Oxide Synthase

nitric oxide synthase, partial; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III

NOS

NOS3; NOS3; eNOS; ECNOS; cNOS; eNOS; NOSIII

NOS3_HUMAN

Rat

Store all reagents at 2-8 degree C

ELISA Type: Sandwich

4262036

AAD14336.1

P29474

133,289 Da

ACE Inhibitor Pathway 198763!!AGE/RAGE Pathway 698754!!Angiogenesis Pathway 198772!!Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Disease Pathway 530764!!Endothelin Pathway 672453

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Ref.7Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Ref.7. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Ref.20 Ref.21. Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN. Ref.20 Ref.21. Subcellular location: Cell membrane. Membrane caveola. Cytoplasm cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Ref.20 Ref.21 Ref.23 Ref.24. Tissue specificity: Platelets, placenta, liver and kidney. Ref.19. Post-translational modification: Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity . By similarity. Phosphorylation of Ser-114 by CDK5 reduces activity. Polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

96 Strip Wells

600 USD