ELISA/assay

Rat Catalase ELISA Kit

MBS006963

48-Strip-Wells

435 USD

ELISA Kit

Rat Catalase ELISA Kit

Catalase

catalase; Catalase; catalase; catalase

CAT

CAT; CAT

CATA_HUMAN

Rat

No significant cross-reactivity or interference between Rat CAT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 U/ml - 200 U/ml. Sensitivity: 1.0 U/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CAT concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CAT and CAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CAT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CAT concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CAT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CAT and CAT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557014

NP_001743.1

NM_001752.3

P04040

59,756 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); DNA Damage Response (only ATM Dependent) Pathway (198827); Folate Metabolism Pathway (198833); FoxO Family Signaling Pathway (138036); Glyoxylate And Dicarboxylate Metabolism Pathway (83002); Glyoxylate And Dicarboxylate Metabolism Pathway (383); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Oxidative Stress Pathway (198916)

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family. Protein type: Hydrolase; Amino Acid Metabolism - tryptophan; Energy Metabolism - methane; Endoplasmic reticulum; Mitochondrial; EC 1.11.1.6; Oxidoreductase; Apoptosis. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: Golgi apparatus; peroxisomal membrane; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol. Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; enzyme binding; catalase activity; metal ion binding; heme binding; NADP binding; receptor binding; aminoacylase activity. Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; protein homotetramerization; activation of NF-kappaB transcription factor; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to reactive oxygen species; response to vitamin E; triacylglycerol metabolic process; response to hyperoxia; UV protection; inhibition of NF-kappaB transcription factor; ureteric bud development; hydrogen peroxide catabolic process; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; aging; negative regulation of apoptosis. Disease: Acatalasemia

48-Strip-Wells

435 USD

96-Strip-Wells

600