catalog number :
MBS006597
products type :
ELISA Kit
products full name :
Human 4-Hydroxynonenal ELISA Kit
products short name :
4-Hydroxynonenal
products gene name :
4-HNE
specificity :
No significant cross-reactivity or interference between Human 4-HNE and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 mumol/L - 100 mumol/L. Sensitivity: 1.0 umol/L.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 4-HNE concentrations in Human serum, plasma and other body fluids. Using Purified Human 4-HNE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 4-HNE and 4-HNE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 4-HNE in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 4-HNE concentrations in Human serum, plasma and other body fluids. Using Purified Human 4-HNE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 4-HNE and 4-HNE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 4-HNE in the samples is then determined by comparing the O.D. of the samples to the standard curve.