ELISA/assay

Porcine Insulin Like Growth Factor1 ELISA Kit

MBS006210

48-Strip-Wells

435 USD

ELISA Kit

Porcine Insulin Like Growth Factor1 ELISA Kit

Insulin Like Growth Factor1

insulin-like growth factor 1 isoform 3 preproprotein; Insulin-like growth factor I; insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; insulin-like growth factor 1 (somatomedin C); Mechano growth factor; MGF; Somatomedin-C

IGF1

IGF1; IGF1; IGFI; IGF-I; IGF1A; IBP1; IGF-I; MGF

IGF1_HUMAN

Porcine

No significant cross-reactivity or interference between Porcine IGF1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGF1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine IGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGF1 and IGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGF1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine IGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGF1 and IGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

163659903

NP_001104755.1

NM_001111285.1

P05019

21,841 Da

Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198812); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding. Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; exocrine pancreas development; glycolate metabolic process; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of mitosis; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; positive regulation of activated T cell proliferation; signal transduction; positive regulation of smooth muscle cell migration; negative regulation of cell proliferation; glial cell differentiation; platelet degranulation; mammary gland development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; regulation of multicellular organism growth; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of osteoblast differentiation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; Ras protein signal transduction; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell motility; blood coagulation; positive regulation of DNA replication. Disease: Insulin-like Growth Factor I Deficiency

48-Strip-Wells

435 USD

96-Strip-Wells

600