ELISA/assay

Rabbit Insulin ELISA Kit

MBS005989

96 Strip Wells

600 USD

ELISA Kit

Rabbit Insulin ELISA Kit

Insulin

insulin; Insulin; insulin; proinsulin; preproinsulin; insulin

INS

INS; INS; ILPR; IRDN; IDDM2; MODY10

INS_HUMAN

Rabbit

No significant cross-reactivity or interference between Rabbit INS and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated INS concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit INS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add INS and INS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 3.12 mU/L – 100 mU/L.

Sensitivity: The sensitivity of this kit is 1.0 mU/L. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated INS concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit INS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add INS and INS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

386828

AAA59172.1

P01308

11,981 Da

AGE/RAGE Pathway 698754!!ATF-2 Transcription Factor Network Pathway 138006!!Adipogenesis Pathway 198832!!Aldosterone-regulated Sodium Reabsorption Pathway 130626!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Amyloids Pathway 366238!!Arf6 Trafficking Events Pathway 137954!!Cardiac Progenitor Differentiation Pathway 712094!!Developmental Biology Pathway 477129!!Disease Pathway 530764

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Subunit structure: Heterodimer of a B chain and an A chain linked by two disulfide bonds. Subcellular location: Secreted. Involvement in disease: Hyperproinsulinemia, familial (FHPRI) [MIM:176730]: An autosomal dominant condition characterized by elevated levels of serum proinsulin-like material.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.21 Ref.23 Ref.24 Ref.25Diabetes mellitus, insulin-dependent, 2 (IDDM2) [MIM:125852]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.34Diabetes mellitus, permanent neonatal (PNDM) [MIM:606176]: A rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32 Ref.33Maturity-onset diabetes of the young 10 (MODY10) [MIM:613370]: A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.33 Ref.34 Ref.35. Pharmaceutical use: Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53. Sequence similarities: Belongs to the insulin family. Sequence caution: The sequence AAA59179.1 differs from that shown. Reason: Erroneous gene model prediction.

96 Strip Wells

600 USD