ELISA/assay

Bovine Interferon gamma ELISA Kit

MBS005893

48-Strip-Wells

435 USD

ELISA Kit

Bovine Interferon gamma ELISA Kit

Interferon gamma

interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon

IFN-gamma

IFNG; IFNG; IFG; IFI; IFN-gamma

IFNG_HUMAN

Bovine

No significant cross-reactivity or interference between Bovine IFN-gamma and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IFN-gamma concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine IFN-gamma antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IFN-gamma and IFN-gamma antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IFN-gamma concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine IFN-gamma antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IFN-gamma and IFN-gamma antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

56786138

NP_000610.2

NM_000619.2

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Cytokine; Secreted, signal peptide; Membrane protein, integral; Secreted. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; positive regulation of interleukin-1 beta secretion; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

435 USD

96-Strip-Wells

600