Canine Glycoprotein VI, Platelet ELISA Kit | MyBioSource MBS005646 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Glycoprotein VI, Platelet ELISA Kit

catalog :

MBS005646

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS005646

products type :

ELISA Kit

products full name :

Canine Glycoprotein VI, Platelet ELISA Kit

products short name :

Glycoprotein VI, Platelet

other names :

glycoprotein VI, partial; Platelet glycoprotein VI; platelet glycoprotein VI; glycoprotein 6; platelet collagen receptor; glycoprotein VI (platelet); Glycoprotein 6

products gene name :

GP6

other gene names :

GP6; GP6; GPIV; GPVI; BDPLT11; GPVI

uniprot entry name :

GPVI_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine GP6 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GP6 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine GP6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GP6 and GP6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GP6 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine GP6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GP6 and GP6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

26985217

ncbi acc num :

AAN86273.1

uniprot acc num :

Q9HCN6

ncbi mol weight :

36,866 Da

ncbi summary :

This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

GPVI: Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma- chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. Defects in GP6 are the cause of bleeding disorder platelet-type 11 (BDPLT11). BDPLT11 is a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: collagen binding; protein binding; transmembrane receptor activity; receptor activity. Biological Process: platelet activation; blood coagulation; leukocyte migration; enzyme linked receptor protein signaling pathway. Disease: Bleeding Disorder, Platelet-type, 11

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage