Hamster Endothelial Lipase ELISA Kit | MyBioSource MBS005633 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hamster Endothelial Lipase ELISA Kit

catalog :

MBS005633

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS005633

products type :

ELISA Kit

products full name :

Hamster Endothelial Lipase ELISA Kit

products short name :

Endothelial Lipase

other names :

endothelial lipase; Endothelial lipase; endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase; lipase, endothelial; Endothelial cell-derived lipase

products gene name :

other gene names :

LIPG; LIPG; EL; EDL; PRO719; EDL; EL

uniprot entry name :

LIPE_HUMAN

reactivity :

Hamster

specificity :

No significant cross-reactivity or interference between Hamster EL and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 ng/ml - 1000 ng/ml. Sensitivity: 5.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EL concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster EL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EL and EL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EL concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster EL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EL and EL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

5174497

ncbi acc num :

NP_006024.1

ncbi gb acc num :

NM_006033.2

uniprot acc num :

Q9Y5X9

ncbi mol weight :

56,795 Da

ncbi pathways :

Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Triacylglycerol Degradation Pathway (142418)

ncbi summary :

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

uniprot summary :

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Lipid Metabolism - glycerolipid; Secreted, signal peptide; Phospholipase; EC 3.1.1.3. Chromosomal Location of Human Ortholog: 18q21.1. Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome. Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity. Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage