ELISA/assay

Monkey Mast Cell Tryptase ELISA Kit

MBS005180

48-Strip-Wells

435 USD

ELISA Kit

Monkey Mast Cell Tryptase ELISA Kit

Mast Cell Tryptase

mast cell tryptase

MCT

Monkey

No significant cross-reactivity or interference between Monkey MCT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MCT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey MCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MCT and MCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MCT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MCT concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey MCT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MCT and MCT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

556556

BAA07486.1

serine protease; plays a role during the inflammatory process [RGD, Feb 2006]

TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily. Protein type: Protease; EC 3.4.21.59; Secreted; Secreted, signal peptide. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: peptidase activity; heparin binding; serine-type endopeptidase activity. Biological Process: proteolysis

48-Strip-Wells

435 USD

96-Strip-Wells

600