Rabbit BAD (Phospho-Ser91/128) Antibody | MyBioSource MBS004216 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit BAD (Phospho-Ser91/128) Antibody

catalog :

MBS004216

quantity :

0.05 mL

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS004216

products type :

Antibody

products full name :

Rabbit BAD (Phospho-Ser91/128) Antibody

products short name :

BAD (Phospho-Ser91/128)

other names :

bcl2 antagonist of cell death; Bcl2 antagonist of cell death; bcl2 antagonist of cell death; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter; BCL2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter

products gene name :

BAD

other gene names :

BAD; BAD; BBC2; BCL2L8; BBC6; BCL2L8; BAD; Bcl2-L-8

uniprot entry name :

BAD_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

168

specificity :

BAD (Phospho-Ser91/128) antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1 mg/ml

storage stability :

Store at -20 degree C

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500~1:3000 . IHC: 1:50~1:100. ELISA: 1:10000

other info1 :

All Sites: Human: Ser91; Mouse: Ser128; Rat: Ser129. Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 91/ serine 128 (E-L-SP-P-F).

ncbi gi num :

10835069

ncbi acc num :

NP_004313.1

ncbi gb acc num :

NM_004322.3

uniprot acc num :

Q92934

ncbi mol weight :

18,392 Da

ncbi pathways :

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alpha-synuclein Signaling Pathway (137913); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509)

ncbi summary :

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

uniprot summary :

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol. Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding. Biological Process: response to oleate; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; apoptosis; positive regulation of apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; response to estradiol stimulus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; release of cytochrome c from mitochondria; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; positive regulation of insulin secretion; ADP metabolic process; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; induction of apoptosis via death domain receptors; positive regulation of T cell differentiation; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; innate immune response; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; response to progesterone stimulus; response to calcium ion; positive regulation of epithelial cell proliferation

size1 :

0.05 mL

price1 :

270 USD

size2 :

0.1 mL

price2 :

445

more info or order :

MyBioSource product webpage