Rabbit ATF-4 (Phospho-Ser219) Antibody | MyBioSource MBS003653 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit ATF-4 (Phospho-Ser219) Antibody

catalog :

MBS003653

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of ATF-4 (Phospho-Ser219) expression in A549 cells

image 2 :

Western blot analysis of extracts from A549, using Phospho-ATF-4 (Ser219) Ab. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

product information

catalog number :

MBS003653

products type :

Antibody

products full name :

Rabbit ATF-4 (Phospho-Ser219) Antibody

products short name :

[ATF-4 (Phospho-Ser219)]

products name syn :

[Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF4;cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB]

other names :

[cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67; activating transcription factor 4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67]

products gene name :

[ATF-4]

other gene names :

[ATF4; ATF4; CREB2; TXREB; CREB-2; TAXREB67; CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67]

uniprot entry name :

ATF4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, mouse

sequence length :

351

specificity :

ATF-4 (Phospho-Ser219) Antibody detects endogenous levels of total ATF-4 (Phospho-Ser219)

purity :

The Ab is from purified rabbit serum by affinity purificationvia sequential chromatography on phospho- and non-phospho-peptide affinity columns.

form :

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

concentration :

1mg/ml

storage stability :

Store at -20 °C. Stable for 12 months from date of receipt.

tested application :

Western Blot (WB)

app notes :

WB 1:500-1:2000. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking,overnight.

image1 heading :

Western blot (WB)

image2 heading :

Western Blot (WB)

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production.

ncbi gi num :

33469976

ncbi acc num :

NP_001666.2

ncbi gb acc num :

NM_001675.2

uniprot acc num :

P18848

ncbi mol weight :

40 kDa

ncbi pathways :

Activation Of Chaperone Genes By ATF6-alpha Pathway (530770); Activation Of Chaperones By ATF6-alpha Pathway (105905); Activation Of Genes By ATF4 Pathway (530772); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Cholinergic Synapse Pathway (217716); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273)

ncbi summary :

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

uniprot summary :

ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus. Molecular Function: protein C-terminus binding; protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; amino acid metabolic process; unfolded protein response; positive regulation of apoptosis; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; gluconeogenesis; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage