Rabbit AS160 (Phospho-Thr642) Antibody | MyBioSource MBS002191 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit AS160 (Phospho-Thr642) Antibody

catalog :

MBS002191

quantity :

0.05 mL

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS002191

products type :

Antibody

products full name :

Rabbit AS160 (Phospho-Thr642) Antibody

products short name :

AS160 (Phospho-Thr642)

other names :

TBC1 domain family member 4; TBC1 domain family member 4; TBC1 domain family member 4; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family, member 4; Akt substrate of 160 kDa

products gene name :

AS160

other gene names :

TBC1D4; TBC1D4; AS160; AS160; KIAA0603; AS160

uniprot entry name :

TBCD4_HUMAN

host :

Rabbit

reactivity :

Human, mouse

sequence length :

1298

specificity :

Phospho-TBC1D4 (T642) Polyclonal Antibody dectects endogenous levels of TBC1D4 protein only when phosphorylated at T642.

purity :

The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography using epitope-specific immunogen.

form :

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% soidum azide.

concentration :

1 mg/ml

storage stability :

20 Degree C/ 1 year

tested application :

Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

Immunohistochemistry: 1/100-1/300. Immunofluorescence: 1/200-1/1000. ELISA: 1/5000. Not yet tested in other applications.

other info1 :

Immunogen: Synthesized peptide derived from human TBC1D4 around the phoshorylation site of T642.

ncbi gi num :

114688046

ncbi acc num :

NP_055647.2

ncbi gb acc num :

NM_014832.2

uniprot acc num :

O60343

ncbi mol weight :

146,563 Da

ncbi pathways :

Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Insulin Signaling Pathway (198845); Insulin-mediated Glucose Transport Pathway (137981); Membrane Trafficking Pathway (106160); Translocation Of GLUT4 To The Plasma Membrane Pathway (685554)

ncbi summary :

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

uniprot summary :

AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue. Protein type: GAPs; GAPs, Rab. Chromosomal Location of Human Ortholog: 13q22.2. Cellular Component: cytoplasmic vesicle membrane; cytoplasm. Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus. Disease: Diabetes Mellitus, Noninsulin-dependent, 5

size1 :

0.05 mL

price1 :

270 USD

size2 :

0.1 mL

price2 :

445

more info or order :

MyBioSource product webpage