catalog number :
MBS001394
products full name :
Rabbit RAD52 (Ab-104) Antibody
products short name :
[RAD52 (Ab-104)]
other names :
[DNA repair protein RAD52 homolog; DNA repair protein RAD52 homolog; DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23; RAD52 homolog (S. cerevisiae)]
products gene name :
[RAD52]
other gene names :
[RAD52; RAD52]
uniprot entry name :
RAD52_HUMAN
reactivity :
Human, mouse
specificity :
RAD52 (Ab-104) antibody detects endogenous levels of total RAD52 protein.
purity :
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
form :
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
storage stability :
Store at -20°C/1 year
tested application :
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
WB: 1:500~1:1000. IHC: 1:50~1:100. IF: 1:100~1:500. ELISA: 1:10000
image1 heading :
Immunohistochemistry
image2 heading :
Western blot
image3 heading :
Immunofluorescence analysis of COS7 cells, using RAD52 antibody.
other info1 :
Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104 (K-F-YP-V-G).
ncbi acc num :
NP_602296.2
ncbi gb acc num :
NM_134424.2
ncbi mol weight :
46,169 Da
ncbi pathways :
Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway (105873); DNA Repair Pathway (105837); DNA Damage Response Pathway (198788); Double-Strand Break Repair Pathway (105861); Homologous DNA Pairing And Strand Exchange Pathway (105870); Homologous Recombination Repair Pathway (105862); Homologous Recombination Pathway (198890); Homologous Recombination Pathway (83046); Homologous Recombination Pathway (454); Homologous Recombination Repair Of Replication-independent Double-strand Breaks Pathway (105863)
ncbi summary :
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq, Jul 2008]
uniprot summary :
RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family. Protein type: DNA repair, damage. Chromosomal Location of Human Ortholog: 12p13-p12.2. Cellular Component: nucleoplasm; nucleus. Molecular Function: identical protein binding; protein binding; DNA binding. Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination