Rabbit MYPT1 (Phospho-Thr696) Antibody | MyBioSource MBS000751 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit MYPT1 (Phospho-Thr696) Antibody

catalog :

MBS000751

quantity :

0.1 mL

price :

440 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of 293 cells, using Phospho-MYPT1 (Thr696) Ab.

product information

catalog number :

MBS000751

products type :

Antibody

products full name :

Rabbit MYPT1 (Phospho-Thr696) Antibody

products short name :

[MYPT1 (Phospho-Thr696)]

other names :

[protein phosphatase 1 regulatory subunit 12A isoform a; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1 regulatory subunit 12A; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase myosin-binding subunit; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit]

products gene name :

[MYPT1]

other gene names :

[PPP1R12A; PPP1R12A; MBS; M130; MYPT1; MBS; MYPT1; Myosin phosphatase target subunit 1]

uniprot entry name :

MYPT1_HUMAN

clonality :

polyclonal

host :

rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1030

specificity :

Phospho-MYPT1 (Thr696) Ab detects endogenous levels of MYPT1.

purity :

The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and nonphospho- peptide affinity columns.

concentration :

1 mg/ml

storage stability :

Store at -20°C. Stable for 12 months from date of receipt.

app notes :

WB 1:500-1:2000. IHC 1:50-1:200

image1 heading :

Western Blot(WB)

other info1 :

Immunogen: A synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr696. Tissue specifity: Expressed in striated muscles, specifically in type 2a fibers (at protein level). Similarity: Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.The KVKF motif mediates interaction with PPP1CB. Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

other info2 :

Note: For western blot, incubate membrane with diluted Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

ncbi gi num :

219842212

ncbi acc num :

NP_001137357.1

ncbi gb acc num :

NM_001143885.1

uniprot acc num :

O14974

ncbi mol weight :

140 kDa

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); G2/M Transition Pathway (105801); Long-term Potentiation Pathway (83085); Long-term Potentiation Pathway (496); Mitotic G2-G2/M Phases Pathway (160942); PLK1 Signaling Events Pathway (138007)

ncbi summary :

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

uniprot summary :

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described. Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q15-q21. Cellular Component: nucleoplasm; kinetochore; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton. Molecular Function: enzyme inhibitor activity; protein binding; signal transducer activity; phosphatase regulator activity; protein kinase binding. Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; protein amino acid dephosphorylation; signal transduction; G2/M transition of mitotic cell cycle

size1 :

0.1 mL

price1 :

440 USD

size2 :

0.2 mL

price2 :

715

more info or order :

MyBioSource product webpage