Rabbit MYPT1 (Phospho-Thr853) Antibody | MyBioSource MBS000692 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit MYPT1 (Phospho-Thr853) Antibody

catalog :

MBS000692

quantity :

0.05 mL

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS000692

products type :

Antibody

products full name :

Rabbit MYPT1 (Phospho-Thr853) Antibody

products short name :

MYPT1 (Phospho-Thr853)

other names :

protein phosphatase 1 regulatory subunit 12A isoform a; Protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1 regulatory subunit 12A; myosin binding subunit; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase myosin-binding subunit; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit

products gene name :

MYPT1

other gene names :

PPP1R12A; PPP1R12A; MBS; M130; MYPT1; MBS; MYPT1; Myosin phosphatase target subunit 1

uniprot entry name :

MYPT1_HUMAN

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

1030

specificity :

MYPT1 (Phospho-Thr853) antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine 853.

purity :

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg^2+ and Ca^2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1 mg/ml

storage stability :

Store at -20 degree C for 1 year

tested application :

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500~1:1000 . IHC: 1:50~1:100. ELISA: 1:1000

other info1 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MYPT1 around the phosphorylation site of threonine 853 (R-S-TP-G-V).

ncbi gi num :

219842212

ncbi acc num :

NP_001137357.1

ncbi gb acc num :

NM_001143885.1

uniprot acc num :

O14974

ncbi mol weight :

115,281 Da

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); G2/M Transition Pathway (105801); Long-term Potentiation Pathway (83085); Long-term Potentiation Pathway (496); Mitotic G2-G2/M Phases Pathway (160942); PLK1 Signaling Events Pathway (138007)

ncbi summary :

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

uniprot summary :

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described. Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q15-q21. Cellular Component: kinetochore; nucleoplasm; centrosome; focal adhesion; contractile fiber; cytoplasm; actin cytoskeleton. Molecular Function: enzyme inhibitor activity; protein binding; signal transducer activity; phosphatase regulator activity; protein kinase binding. Biological Process: regulation of cell adhesion; mitosis; centrosome organization and biogenesis; regulation of nucleocytoplasmic transport; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; signal transduction; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation

size1 :

0.05 mL

price1 :

270 USD

size2 :

0.1 mL

price2 :

445

more info or order :

MyBioSource product webpage