Rabbit anti CD4 Polyclonal
MyBioSource
catalog: MBS551067
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay
quantity: 0.1 mg
price: 280 USD
to the supplier
Anti-CD3 epsilon antibody
MyBioSource
catalog: MBS355170
domestic rabbit polyclonal
reactivity: human, mouse, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 0.1 mg
price: 285 USD
to the supplier
mAb anti-Human CD3
MyBioSource
catalog: MBS592283
mouse monoclonal (JXT3)
reactivity: human
application: immunocytochemistry
quantity: 0.5 mg
price: 430 USD
to the supplier
Anti-CD3 epsilon Rabbit Monoclonal Antibody
MyBioSource
catalog: MBS179879
domestic rabbit monoclonal ([EED-3])
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS

Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (MBS179879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3E.

IHC analysis of CD3 epsilon using anti- CD3 epsilon antibody (MBS179879) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD3 epsilon Antibody (MBS179879) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.
quantity: 0.03 mg
price: 160 USD
to the supplier
CD3E antibody
MyBioSource
catalog: MBS200286
mouse monoclonal ([AT3H3])
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
quantity: 0.05 mL
price: 240 USD
to the supplier
CD3e (T-Cell Marker) Mouse Monoclonal Antibody [Clone C3e/1308]
MyBioSource
catalog: MBS4380150
mouse monoclonal ([C3e/1308])
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)

SDS-PAGE Analysis Purified CD3 epsilon Monoclonal Antibody (C3e/1308). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of human Jurkat cell lysate using CD3e Monoclonal Antibody (C3e/1308).
quantity: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
price: 190 USD
to the supplier
CD3e (T-Cell Marker)
MyBioSource
catalog: MBS438059
domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chicken, bovine, chimpanzee, rhesus macaque
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Western blot of CD3e in human Jurkat cells . 1) absence . 2) presence of immunizing peptide. 3) Mouse thymus probed with CD3e Rabbit PAb.

Western Blot of lurkat Cell Lysate using CD3e Rabbit Polyclonal Antibody.
quantity: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
price: 190 USD
to the supplier
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
MyBioSource
catalog: MBS439364
mouse monoclonal ([RIV9])
reactivity: human, mouse, rat
application: immunocytochemistry, flow cytometry, FACS

SDS-PAGE Analysis. CD3e Mouse Monoclonal Antibody (RIV9). Confirmation of Integrity and Purity of Antibody

Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9). Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
quantity: 0.02 mg (With BSA & Azide at 0.2 mg/ml)
price: 190 USD
to the supplier
Rabbit anti CD3 Polyclonal
MyBioSource
catalog: MBS551029
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay

IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of FFPE rat kidney sections showing lymphocyte expression of CD3. It was used as primary antibody, dilution 1:200, DAB chromogenic staining.

Immunoflourescence of human colon cancer sections showing expression of CD3. MBS551029 was used as a primary antibody , dilution 1:60.
quantity: 0.1 mg
price: 280 USD
to the supplier