rabbit polyclonal
reactivity: human, mouse, rat, cow, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat, cow, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of STAT1 (pY701) expression in HEK293T (A), NIH3T3 (B), mouse brain (C), COS7 (D) whole cell lysates.
Immunohistochemical analysis of STAT1 (pY701) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of STAT1 (pY701) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 165 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat, cow
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of FOXO1 (pS329) expression in HEK293T (A), SHSY5Y (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
Immunohistochemical analysis of FOXO1 (pS329) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FOXO1 (pS329) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 165 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Western blot analysis of extracts from TSA treated HeLa cells, using Acetyl-FKHR(K294) Ab. Lane 1: Heat-shock treated COS-7, blocked with antigen-specific peptides. Lane 2: Heat-shock treated COS-7. Lane 3: LPS treated A2780 cells. Observed bands: 90 kDa
MBS9600633 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
quantity: 0.1 mL
price: 240 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
