rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
mouse monoclonal (2C11)
reactivity: human
application: western blot, ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay
reactivity: human
application: western blot, ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay
Rabbit anti-human thyroid stimulating hormone receptor polyclonal Antibody
MyBioSource
catalog: MBS710147
MyBioSource
catalog: MBS710147
rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, enzyme immunoassay
reactivity: human, mouse
application: western blot, ELISA, enzyme immunoassay
rabbit polyclonal
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (MBS1750438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
quantity: 0.1 mg
price: 280 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of extracts of various cell lines, using TSHR antibody.
quantity: 0.06 mL
price: 150 USD
to the supplier